Open AccessCombining HPV and MIB‐1 tests reduces the number of colposcopies in women with equivocal cytology
Author(s) -
WENSVEEN C.W.M.,
KAGIE M.J.,
VELDHUIZEN R.W.,
TRIMBOS J.B.M.Z.,
BOON M.E.
Publication year - 2006
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1080/00016340600936001
Subject(s) - colposcopy , medicine , cervical intraepithelial neoplasia , cytology , gynecology , human papillomavirus , cervical cancer , intraepithelial neoplasia , koilocyte , immunostaining , papillomaviridae , obstetrics , pathology , cancer , immunohistochemistry , prostate
Abstract Background. In this study the MIB‐1 immunostaining pattern as an index of cellular proliferation was analyzed in smears diagnosed as borderline dyskaryosis in order to establish whether the combination of human papillomavirus testing and MIB‐1 staining could resolve equivocal cytology. Methods. Conventional Pap smears of 108 women diagnosed as borderline dyskaryosis were stained with MIB‐1 and the proliferation index was assessed. These women were evaluated by colposcopy, histological sampling, and human papillomavirus, semi‐quantitative evaluated by hybrid Capture II test. Results. All 64 human papillomavirus‐ and MIB‐1‐negative women had no underlying high‐grade cervical intraepithelial neoplasia or cervical cancer. Forty of the 104 women with normal histology or cervical intraepithelial neoplasia I were positive for human papillomavirus, compared to only one positive MIB‐1 test (i.e. proliferation index of more than 35%). Conclusions. Adding a MIB‐1‐test in human papillomavirus‐positive women with equivocal cytology might reduce the number of colposcopies needed to predict ≥ cervical intraepithelial neoplasia II. With this approach only four instead of 43 human papillomavirus‐positive women would have been referred for colposcopy.