Open AccessChip-based genotyping by mass spectrometry
Author(s) -
Kai Tang,
Dong-Jing Fu,
Dominique Julien,
Andreas Braun,
Charles R. Cantor,
Hubert Köster
Publication year - 1999
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.96.18.10016
Subject(s) - mass spectrometry , genotyping , chemistry , primer extension , chromatography , chip , desorption , analytical chemistry (journal) , dna , base sequence , genotype , computer science , biochemistry , adsorption , gene , telecommunications , organic chemistry
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells viaN -succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-μl scale directly in the chip wells in parallel. Diagnostic products thus generated were detectedin situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.