Open AccessAdult fast myosin pattern and Ca 2+ -induced slow myosin pattern in primary skeletal muscle culture
Author(s) -
HansPeter Kubis,
Ernst-August Haller,
Petra Wetzel,
Gerolf Gros
Publication year - 1997
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.94.8.4205
Subject(s) - myogenesis , myosin , microcarrier , ionophore , myocyte , biology , lactate dehydrogenase , intracellular , microbiology and biotechnology , biochemistry , skeletal muscle , biophysics , endocrinology , enzyme , cell , membrane
A primary muscle cell culture derived from newborn rabbit muscle and growing on microcarriers in suspension was established. When cultured for several weeks, the myotubes in this model develop the completely adult pattern of fast myosin light and heavy chains. When Ca2+ ionophore is added to the culture medium on day 11, raising intracellular [Ca2+ ] about 10-fold, the myotubes develop to exhibit properties of an adult slow muscle by day 30, expressing slow myosin light as well as heavy chains, elevated citrate synthase, and reduced lactate dehydrogenase. The remarkable plasticity of these myotubes becomes apparent, when 8 days after withdrawal of the ionophore a marked slow-to-fast transition, as judged from the expression of isomyosins and metabolic enzymes, occurs.