A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3′ end | Zendy

Herbert Tschochner | Zendy

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A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3′ end

Author(s) -

Herbert Tschochner

Publication year - 1996

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.93.23.12914

Subject(s) - termination factor , rna polymerase ii , microbiology and biotechnology , rnase h , rna , biology , transcription (linguistics) , rnase p , transcription factor ii e , polymerase , rna polymerase i , dna , rna polymerase ii holoenzyme , rna dependent rna polymerase , genetics , gene , gene expression , promoter , linguistics , philosophy

A novel RNase activity was identified in a yeast RNA polymerase I (pol I)in vitro transcription system. Transcript cleavage occurred at the 3′ end and was dependent on the presence of ternary pol I/DNA/RNA complexes and an additional protein factor not identical to transcription factor IIS (TFIIS). Transcript cleavage was observed both on arrested complexes at the linearized ends of the transcribed DNA and on intrinsic blocks of the DNA template. Shortened transcripts that remained associated within the ternary complexes were capable of resuming RNA chain elongation. Possible functions of the nuclease for transcript elongation or termination are discussed.

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