Open AccessYY1 and c-Myc associate in vivo in a manner that depends on c-Myc levels.
Author(s) -
Ajay Shrivastava,
Jin Chen Yu,
Steven E. Artandi,
Kathryn Calame
Publication year - 1996
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.93.20.10638
Subject(s) - yy1 , transcription factor , transcription (linguistics) , proto oncogene proteins c myc , transcription factor ii b , biology , dna binding protein , repressor , microbiology and biotechnology , general transcription factor , regulator , gene , promoter , gene expression , biochemistry , linguistics , philosophy
The c-Myc oncoprotein has previously been shown to associate with transcription regulator YY1 and to inhibit its activity. We show herein that endogenous c-Myc and YY1 associate in vivo and that changes in c-Myc levels, which accompany mitogenic stimulation or differentiation of cultured cells, affect the ratio of free to c-Myc-associated YY1. We have also investigated the mechanism by which association with c-Myc inhibits YY1's ability to regulate transcription. c-Myc does not block binding of YY1 to DNA. However, protein association studies suggest that c-Myc interferes with the ability of YY1 to contact basal transcription proteins TATA-binding protein and TFIIB.