Open AccessAlternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor.
Author(s) -
Raffaella Calligaris,
Stefania Bottardi,
Susanna Cogoi,
Isabel Apezteguia,
Claudio Santoro
Publication year - 1995
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.92.25.11598
Subject(s) - transactivation , zinc finger , transcription factor , biology , gata transcription factor , gata2 , start codon , gata4 , haematopoiesis , microbiology and biotechnology , translation (biology) , messenger rna , genetics , gene , gene expression , stem cell , promoter
GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.