Open AccessHuman TAFII31 protein is a transcriptional coactivator of the p53 protein.
Author(s) -
Hua Lu,
Arnold J. Levine
Publication year - 1995
Publication title -
proceedings of the national academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.92.11.5154
Subject(s) - taf2 , transcription factor ii d , general transcription factor , transcription factor ii a , coactivator , taf1 , taf4 , rna polymerase ii , tata box binding protein , biology , tata binding protein , microbiology and biotechnology , gatad2b , transcription factor ii b , transcription (linguistics) , transcription factor , dna binding protein , biochemistry , rna polymerase , rna , gene , promoter , gene expression , repressor , enhancer , linguistics , philosophy
The p53 protein activates transcription of a target gene by binding to a specific DNA response element and interacting with the transcriptional apparatus of RNA polymerase II. The amino-terminal domain of p53 interacts with a component of the TFIID basal transcription complex. The human TATA-binding-protein-associated factor TAFII31, a component of TFIID, has been identified as a critical protein required for p53-mediated transcriptional activation. TAFII31 and p53 proteins bind to each other via amino acid residues in the amino-terminal domain of p53 that are essential for transcription. Antibodies directed against TAFII31 protein inhibit p53-activated but not basal transcription in vitro. These results demonstrate that TAFII31 is a coactivator for the p53 protein.
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