Open AccessCombinatorially selected guanosine-quartet structureis a potent inhibitor of human immunodeficiency virus envelope-mediated cellfusion.
Author(s) -
Jacqueline R. Wyatt,
Timothy A. Vickers,
Joseph L. Roberson,
Robert W. Buckheit,
Thomas Klimkait,
Elizabeth L. DeBaets,
Peter W. Davis,
Bernard Rayner,
J.L. Imbach,
David J. Ecker
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.4.1356
Subject(s) - guanosine , tetramer , oligonucleotide , biology , in vitro , virology , v3 loop , virus , microbiology and biotechnology , biochemistry , peptide sequence , gene , enzyme
The phosphorothioate oligonucleotide T2G4T2 wasidentified as an inhibitor of HIV infection in vitro by combinatorial screeningof a library of phosphorothioate oligonucleotides that contained all possibleoctanucleotide sequences. The oligonucleotide forms a parallel-strandedtetrameric guanosine-quartet structure. Tetramer formation and thephosphorothioate backbone are essential for antiviral activity. The tetramerbinds to the human immunodeficiency virus envelope protein gp120 at the V3 loopand inhibits both cell-to-cell and virus-to-cell infection.