Open AccessGalanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy.
Author(s) -
Ru-Rong Ji,
Qin Zhang,
Katarina Bedecs,
Jan Arvidsson,
Xu Zhang,
XiaoJun Xu,
Zsuzsanna WiesenfeldHallin,
Tamàs Bartfai,
Tomas Hökfelt
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.26.12540
Subject(s) - axotomy , autotomy , galanin , sciatic nerve , in situ hybridization , neuropeptide , biology , medicine , chemistry , endocrinology , messenger rna , microbiology and biotechnology , anatomy , central nervous system , biochemistry , receptor , gene
Antisense (AS) oligonucleotides (ONs) to galanin (GAL) were applied to the proximal end of a transected sciatic nerve, allowing their cellular uptake and transport into injured axons. GAL expression in dorsal root ganglia and self-mutilation behavior (autotomy) were then studied. AS-ONs with phosphorothioate or allyl modifications significantly suppressed the axotomy-induced increase in GAL levels, as demonstrated by immunohistochemistry and exaggerated autotomy behavior, whereas no significant effect on GAL mRNA levels could be demonstrated with in situ hybridization. Allyl-ONs were more effective than phosphorothioate-ONs. An AS-ON with three base mismatches did not induce any of the above effects. These results support the view that the inhibition of axotomy-induced GAL up-regulation is related to autotomy.