Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy. | Zendy

RuRong Ji | Zendy; Q Zhang | Zendy; Katarina Bedecs | Zendy; Jan Arvidsson | Zendy; X Zhang | Zendy; Xingtao Xu | Zendy; Z. WiesenfeldHallin | Zendy; Tamás Bartfai | Zendy; T Hökfelt | Zendy

Open Access

Galanin antisense oligonucleotides reduce galanin levels in dorsal root ganglia and induce autotomy in rats after axotomy.

Author(s) -

RuRong Ji,

Q Zhang,

Katarina Bedecs,

Jan Arvidsson,

X Zhang,

Xingtao Xu,

Z. WiesenfeldHallin,

Tamás Bartfai,

T Hökfelt

Publication year - 1994

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.91.26.12540

Subject(s) - axotomy , autotomy , galanin , sciatic nerve , in situ hybridization , neuropeptide , biology , medicine , chemistry , endocrinology , messenger rna , microbiology and biotechnology , anatomy , central nervous system , biochemistry , receptor , gene

Antisense (AS) oligonucleotides (ONs) to galanin (GAL) were applied to the proximal end of a transected sciatic nerve, allowing their cellular uptake and transport into injured axons. GAL expression in dorsal root ganglia and self-mutilation behavior (autotomy) were then studied. AS-ONs with phosphorothioate or allyl modifications significantly suppressed the axotomy-induced increase in GAL levels, as demonstrated by immunohistochemistry and exaggerated autotomy behavior, whereas no significant effect on GAL mRNA levels could be demonstrated with in situ hybridization. Allyl-ONs were more effective than phosphorothioate-ONs. An AS-ON with three base mismatches did not induce any of the above effects. These results support the view that the inhibition of axotomy-induced GAL up-regulation is related to autotomy.

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