Open AccessPhysical association of JAK1 and JAK2 tyrosine kinases with the interleukin 2 receptor beta and gamma chains.
Author(s) -
Nobuyuki Tanaka,
Hironobu Asao,
Kazuyuki Ohbo,
Naoto Ishii,
Toru Takeshita,
Masataka Nakamura,
Hidetada Sasaki,
Kazuo Sugamura
Publication year - 1994
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.91.15.7271
Subject(s) - tyrosine kinase , janus kinase 1 , phosphorylation , receptor tyrosine kinase , kinase , tyrosine phosphorylation , janus kinase 2 , signal transduction , tyrosine , protein tyrosine phosphatase , microbiology and biotechnology , chemistry , janus kinase , biology , biochemistry
The functional interleukin 2 (IL-2) receptors contain the beta and gamma chains which are necessary for the transduction of cell growth signals. Monoclonal antibodies specific for the beta chain and gamma chain coimmunoprecipitated JAK1 and 114-kDa JAK2 tyrosine kinases, respectively. Tyrosine phosphorylation of JAK1 and JAK2 was induced upon IL-2 stimulation, and IL-2 activated the JAK2 kinase. These results demonstrate that the JAK1 and JAK2 tyrosine kinases are physically associated with the beta chain and gamma chain, respectively, and suggest that regulation of the kinases may be linked to IL-2-induced signal transduction.