Open AccessThe initiator directs the assembly of a transcription factor IID-dependent transcription complex.
Author(s) -
Juan M. Cárcamo,
Leonard Buckbinder,
Danny Reinberg
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.18.8052
Subject(s) - transcription factor ii d , transcription factor ii a , general transcription factor , taf2 , transcription factor ii b , rna polymerase ii , promoter , tata box , transcription factor ii f , transcription factor ii e , tata binding protein , taf1 , tata box binding protein , rna polymerase ii holoenzyme , biology , microbiology and biotechnology , transcription preinitiation complex , transcription (linguistics) , response element , transcription factor , genetics , gene , gene expression , linguistics , philosophy
Highly purified RNA polymerase II was found to be able to weakly recognize the initiator (Inr) present in the adenovirus IVa2 and major late promoters. The association of RNA polymerase II with the Inr was enhanced by the general transcription factors. The Inr was capable of directing the formation of a DNA-protein complex. Transcription competent complexes on the adenovirus major late and IVa2 promoters appear to be formed by alternative pathways mediated through the Inr and/or "TATA" motif. The presence of both motifs, however, is required for efficient transcription utilizing a discrete start site. Complexes formed at either site required transcription factor TFIID, the TATA binding protein. Consistent with this observation, a TFIID requirement was demonstrated for transcription from a mutant adenovirus major late promoter construct lacking a functional TATA motif.