Open AccessChange in the expression of a nuclear matrix-associated protein is correlated with cellular transformation.
Author(s) -
Claudio Brancolini,
Claudio Schneider
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.16.6936
Subject(s) - nucleoplasm , nucleolus , nuclear matrix , nuclear protein , 3t3 cells , biology , microbiology and biotechnology , immunofluorescence , cytoplasm , cell culture , antibody , genetics , gene , transcription factor , transfection , chromatin
We have identified a monoclonal antibody that recognizes a nuclear matrix-associated protein in NIH 3T3 cells. The immunofluorescence pattern consists predominantly of bright nuclear granule clusters distributed throughout the nucleoplasm, with the exclusion of nucleoli. It recognizes a protein of 190 kDa that is down-regulated to various degrees in a panel of single-oncogene-transformed NIH 3T3 cells. Its localization is similar, but not identical, to the spliceosomal speckles. p190 shows a coordinate expression during the growth cycle of nontransformed NIH 3T3 cells; it is synthesized at the highest level under growth arrest conditions. It is expressed in adult mouse brain and is also present in human IMR-90 fibroblasts.