Open AccessDrosophila retrotransposon promoter includes an essential sequence at the initiation site and requires a downstream sequence for full activity.
Author(s) -
Kevin A. Jarrell,
Matthew Meselson
Publication year - 1991
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.88.1.102
Subject(s) - retrotransposon , promoter , biology , long terminal repeat , genetics , transcription (linguistics) , rna polymerase ii , gene , tata box , rna polymerase iii , consensus sequence , transcription factor ii d , microbiology and biotechnology , rna polymerase , rna , transposable element , gene expression , base sequence , genome , linguistics , philosophy
We describe a 98-base-pair region (-38 to +60) in the long terminal repeat of the Drosophila gypsy retrotransposon that is sufficient for accurate normal-level transcription. We find that, unlike most RNA polymerase II (pol II) promoters, the gypsy promoter includes downstream sequences that are required for full activity. Also unlike most pol II promoters, the gypsy promoter, which lacks a TATA motif, was found to have an essential sequence at the transcription initiation site, mutation of which abolishes transcription. These three uncommon features of the gypsy promoter may be characteristic of a subset of pol II promoters, exemplified by certain retrotransposons and developmental genes of Drosophila and by Tdt, the mouse terminal deoxynucleotidyl-transferase (TdT) gene.