Open AccessUse of synthetic oligonucleotides for genomic DNA dot hybridization to split the DQw3 haplotype.
Author(s) -
Marı́a Martell,
Isabelle Le Gall,
P Millasseau,
J Dausset,
Daniel Cohen
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.8.2682
Subject(s) - oligonucleotide , bamhi , biology , haplotype , genomic dna , genetics , population , dna , microbiology and biotechnology , nucleic acid thermodynamics , gene , genome , genotype , base sequence , demography , sociology
Comparison of two different HLA-DQ beta gene sequences from two DR4 individuals, probably corresponding to DQw3.2 (DQR4) and DQw3.1 (DQR5) specificities, has shown several nucleotide variations. Eight oligonucleotides (24 bases long), derived from these polymorphic areas, have been synthesized. Each oligonucleotide was hybridized to BamHI-digested DNA samples from eight families with HLA-DR4 individuals. Four polymorphic BamHI fragments were detected. Two of eight oligonucleotides gave a single signal (8.9 kilobases) on DQw3.2-positive haplotypes. We used one of these oligonucleotides in a genomic DNA dot hybridization and detected a hybridization signal only in DQw3.2-positive individuals. A very simple test like this allows the screening of a large population sample within a very short period.