Open AccessNeoplastic transformation inactivates specific trans-acting factor(s) required for the expression of the thyroglobulin gene.
Author(s) -
Vittorio Enrico Avvedimento,
Anna Maria Musti,
Alfredo Fusco,
Ian Marc Bonapace,
Roberto Di Lauro
Publication year - 1988
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.85.6.1744
Subject(s) - thyroglobulin , reporter gene , thyroid transcription factor 1 , microbiology and biotechnology , transfection , rous sarcoma virus , thyroid , biology , gene expression , fusion gene , promoter , transcription factor , gene , endocrinology , biochemistry
The expression of rat thyroglobulin gene is repressed following the transformation of rat thyroid cells with Kirsten murine sarcoma virus. The expression of a reporter gene fused to the thyroglobulin promoter is down-regulated in transformed thyroid cells in transient or in stable transfection assays. DNase and exonuclease III cleavage-protection analysis reveals that a promoter binding activity located at -60 base pairs from the transcription start site is substantially reduced in transformed thyroid cells. The repression in the transformed cells of the reporter gene joined to the thyroglobulin promoter can be reversed by fusion with normal differentiated thyroid cells. Fusion of transformed thyroid cells to liver cells does not reactivate the reporter under control of the thyroglobulin promoter.