Open AccessDNA methylation and regulation of the human beta-globin-like genes in mouse erythroleukemia cells containing human chromosome 11.
Author(s) -
Timothy J. Ley,
YaWen Chiang,
D Haidaris,
Nicholas P. Anagnou,
Vincent L. Wilson,
W.F. Anderson
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.21.6618
Subject(s) - biology , dna methylation , gene , microbiology and biotechnology , dna , chromosome , globin , human genome , methylation , genetics , gene expression , genome
The human beta-globin gene is expressed--but the human fetal (gamma) and embryonic (epsilon) globin genes are not--in an induced mouse erythroleukemia cell line (M11-X) that contains most of human chromosome 11. A 24-hr exposure of M11-X cells to 5-azacytidine before induction causes "global" DNA hypomethylation but selective activation of the human gamma-globin genes. Genomic DNA is remethylated 2-3 days after exposure to 5-azacytidine, but sequences near the human and mouse globin genes remain hypomethylated, suggesting that the remethylation process is inhibited in these regions.