Open AccessMolecular cloning and characterization of DNA sequences encoding rat and human atrial natriuretic factors.
Author(s) -
Robert A. Zivin,
Jon H. Condra,
Richard Dixon,
Nabil G. Seidah,
Michel Chrétien,
Moemer,
Michel Chamberland,
Jacques Drouin
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.20.6325
Subject(s) - complementary dna , molecular cloning , peptide sequence , open reading frame , biology , nucleic acid sequence , signal peptide , homology (biology) , amino acid , microbiology and biotechnology , dna , npr2 , escherichia coli , cloning (programming) , biochemistry , gene , natriuretic peptide , medicine , heart failure , computer science , programming language
A cDNA copy of the message encoding rat atrial natriuretic factor (ANF) has been cloned in Escherichia coli, and its nucleotide sequence was determined. ANF appears to be synthesized as a larger precursor, atrial pronatriodilatin. The cDNA has an open reading frame potentially encoding a protein of 152 amino acids, of which the first 24 amino acids strongly resemble a signal sequence. This is followed by a sequence with 80% homology to a second vasoactive protein, porcine cardiodilatin. The ANF peptide is contained in the COOH-terminal portion of the protein. The DNA sequence corresponding to human ANF is also presented and displays a high degree of homology to its rat counterpart. These data provide further evidence for the expression in cardiac atria of a multifactor system that may contribute to the regulation of blood pressure and extracellular fluid volume.