z-logo
open-access-imgOpen Access
Identification and expression in Escherichia coli of merozoite stage-specific genes of the human malarial parasite Plasmodium falciparum.
Author(s) -
Michael J. McGarvey,
Esmail Sheybani,
M Loche,
Luc Perrin,
Bernard Mach
Publication year - 1984
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.81.12.3690
Subject(s) - biology , plasmodium falciparum , gene , complementary dna , cloning (programming) , expression cloning , cdna library , coding region , escherichia coli , recombinant dna , virology , microbiology and biotechnology , genetics , malaria , immunology , computer science , programming language
The key steps in the development of a malaria vaccine through gene cloning are the identification of the proteins involved in host protective immunity and the cloning, identification, and expression of the genes coding for these proteins. Recent data have indicated that certain proteins synthesized at the late schizont-merozoite stage of Plasmodium falciparum play a major role in malaria immunity. This paper reports the identification, in a cDNA library, of recombinant clones corresponding to genes expressed specifically during the late schizont-merozoite stage of P. falciparum development. The 132 cDNA clones thus identified out of 10,000 were found to correspond to only 12 different genes, probably representing most of the major schizont-merozoite specific genes. The stage-specific cDNAs can be efficiently expressed in Escherichia coli cells. The protein products of some of these clones are recognized by monoclonal antibodies specific for late schizont-merozoite proteins. We conclude that only a small set of genes is specifically induced in the schizont-merozoite stage and that the stage-specific cDNA clones we have isolated are very likely to include the genes coding for the immunologically relevant proteins of P. falciparum.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here