Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1. | Zendy

Sylvie van der Werf | Zendy; Czeslaw Wychowski | Zendy; P. Bruneau | Zendy; B Blondel | Zendy; R Crainic | Zendy; F Horodniceanu | Zendy; Marc Girard | Zendy

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Localization of a poliovirus type 1 neutralization epitope in viral capsid polypeptide VP1.

Author(s) -

Sylvie van der Werf,

Czeslaw Wychowski,

P. Bruneau,

B Blondel,

R Crainic,

F Horodniceanu,

Marc Girard

Publication year - 1983

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.80.16.5080

Subject(s) - capsid , poliovirus , virology , plasmid , biology , epitope , escherichia coli , neutralization , microbiology and biotechnology , pbr322 , fusion protein , recombinant dna , complementary dna , virus , peptide sequence , antibody , gene , genetics

Poliovirus type 1 cDNA sequences coding for viral capsid polypeptide VP1 were inserted into the beta-lactamase sequence of Escherichia coli plasmid pBR322. Resulting recombinant plasmid pSW119 expressed in Escherichia coli a VP1-beta-lactamase fusion protein that reacted with antibodies raised against poliovirus capsid polypeptide VP1 and with a monoclonal poliovirus type 1 neutralizing antibody, C3. Deletions of various lengths were generated within the VP1 sequence. The hybrid proteins expressed by the deleted plasmids did not react any more with C3 when the region of VP1 amino acids 95-110 (poliovirus nucleotides 2,754-2,806) was deleted. Therefore, the C3 epitope responsible for virus neutralization is most probably located in this region of the capsid polypeptide.

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