Open AccessRecombination within and between the human insulin and beta-globin gene loci.
Author(s) -
Roger V. Lebo,
Aravinda Chakravarti,
Kenneth H. Buetow,
Michael K. Cheung,
Howard M. Cann,
Barbara Cordell,
Howard M. Goodman
Publication year - 1983
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.80.15.4808
Subject(s) - centimorgan , genetics , biology , recombination , gene , globin , genetic linkage , gene conversion , population , gene mapping , chromosome , demography , sociology
We detected a large number of polymorphic insulin restriction fragments in black Americans. These different size fragments were probably generated by unequal recombination on both sides of the human insulin gene. Population genetic analysis indicates that recombination occurred 33 times more frequently than expected to generate this large number of polymorphic fragments. Specific properties of the unique repeated 14- to 16-base-pair sequences 5' to the insulin gene suggest that this sequence would promote increased unequal recombination. Additional pedigree analysis showed that the recombination rate between the structural insulin and beta-globin gene loci was 14% with strong evidence for linkage. Since both insulin and beta-globin have been mapped to the short arm of human chromosome 11, this study establishes that the genetic map distance between these genes is 14.2 centimorgans.