Open AccessMolecular cloning and physical mapping of varicella-zoster virus DNA.
Author(s) -
Stephen E. Straus,
John D. Owens,
William T. Ruyechan,
Howard Takiff,
Thomas A. Casey,
George F. Vande Woude,
John Hay
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.4.993
Subject(s) - ecori , bamhi , restriction enzyme , microbiology and biotechnology , biology , genome , restriction map , dna , varicella zoster virus , exonuclease , southern blot , genetics , molecular cloning , virology , nucleic acid sequence , virus , gene , complementary dna , dna polymerase
Varicella-zoster virus (VZV) DNA was cleaved with restriction endonuclease EcoRI, and most of the resulting fragments were successfully cloned in the phage vector lambda gtWES . lambda B. Double digestions of cloned fragments with EcoRI and BamHI and hybridizations to blot-transferred BamHI digests of VZV DNA were used to construct a physical map of the genome. The molecular termini of the DNA were identified by restriction enzyme analysis after exonuclease III digestion. The data indicate that VZV DNA exists in two isomeric forms that differ by inversion of one short terminal genome segment. Electron microscopic studies revealed that the short genome segment consists of a terminal revealed that the short genome segment consists of a terminal sequence of about 3.4 X 10(6) daltons that is separated from an internal inverted repeat of itself by a 5.8 X 10(60)-dalton unique DNA segment.