Open AccessConservative integration of bacteriophage Mu DNA into pBR322 plasmid.
Author(s) -
Jean-Claude Liébart,
P. Ghelardini,
L. Paolozzi
Publication year - 1982
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.79.14.4362
Subject(s) - plasmid , pbr322 , bacteriophage mu , bacteriophage , dna , genetics , lysogenic cycle , biology , in vitro recombination , dna replication , plasmid preparation , strain (injury) , microbiology and biotechnology , escherichia coli , gene , molecular cloning , anatomy , peptide sequence
In order to clarify the first step in Mu integrative recombination, we have infected a bacterial strain harboring the plasmid pBR322 and isolated Mu DNA in a supercoiled form associated with this plasmid. These structures show an association of Mu with PBR322 without any preliminary replication.