Open AccessUltraviolet light action spectra for neoplastic transformation and lethality of Syrian hamster embryo cells correlate with spectrum for pyrimidine dimer formation in cellular DNA.
Author(s) -
Jay Doniger,
Elizabeth Jacobson,
Kenneth Krell,
Joseph A. DiPaolo
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.4.2378
Subject(s) - pyrimidine dimer , action spectrum , hamster , neoplastic transformation , transformation (genetics) , dna , dimer , embryo , biology , chinese hamster , mode of action , carcinogenesis , chemistry , biophysics , microbiology and biotechnology , dna damage , genetics , biochemistry , cancer , gene , organic chemistry
Action spectra were determined for neoplastic transformation, production of pyrimidine dimers, and lethality in Syrian hamster embryo cells. Of wavelengths between 240 and 313 nm, the most effective were 265 and 270. The relative sensitivities per quantum for transformation, pyrimidine dimer production, and lethality were essentially the same at each of the wavelengths tested. This action spectrum for transformation, which is relevant to carcinogenesis, is similar to spectra obtained previously by measuring other cellular responses in either microbial or mammalian systems. Because the action spectra for cytotoxicity and transformation are the same as the spectrum for dimer production, DNA is suggested as the target for all these processes.