Open AccessSpecific in vitro initiation of transcription on the adenovirus type 2 early and late EII transcription units.
Author(s) -
Diane Mathis,
R. Elkaïm,
Claude Kédinger,
Paolo Sassone–Corsi,
Pierre Chambon
Publication year - 1981
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.78.12.7383
Subject(s) - transcription (linguistics) , microbiology and biotechnology , biology , rna polymerase ii , general transcription factor , transcription factor ii d , promoter , lytic cycle , e box , dna , gene , gene expression , genetics , virus , philosophy , linguistics
Three transcription units are present in the adenovirus type 2 region EII. Transcription units EIIaE and EIIaL encode the mRNA for the 72,000-dalton DNA binding protein, early and late in the lytic cycle, respectively, and transcription unit EIIb encodes the mRNA for the protein that binds to the 5' termini of adenovirus DNA. By using a cell-free transcription system in the presence of purified RNA polymerase B (or II), we have obtained specific initiation of transcription from both the EIIaE promoter, which does not contain a T-A-T-A box, and the EIIaL promoter, which does. In addition, we have identified a new EII T-A-T-A box promoter that is located close to the early non-T-A-T-A box promoter and is used both in vivo and in vitro.