Expression of the human fibroblast interferon gene in Escherichia coli. | Zendy

Tadatsugu Taniguchi | Zendy; L Guarente | Zendy; Thomas M. Roberts | Zendy; David Kimelman | Zendy; John Douhan | Zendy; Mark Ptashne | Zendy

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Expression of the human fibroblast interferon gene in Escherichia coli.

Author(s) -

Tadatsugu Taniguchi,

L Guarente,

Thomas M. Roberts,

David Kimelman,

John Douhan,

Mark Ptashne

Publication year - 1980

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.77.9.5230

Subject(s) - escherichia coli , plasmid , gene , biology , enterobacteriaceae , fibroblast , microbiology and biotechnology , bacteria , gene expression , interferon , cell culture , genetics

We applied the method of Guarente et al. [Guarente, L., Lauer, G., Roberts, T.M. & Ptashne, M. (1980) Cell 20, 543-553] to construct plasmids that direct expression in Escherichia coli of the human fibroblast interferon (F-IF) gene. Two plasmids were recovered. One directs efficient synthesis of a protein whose primary sequence is that of pre-F-IF and the other, that of mature F-IF. Extracts of bacteria synthesizing mature F-IF display antiviral activity characteristic of human F-IF. This activity is lower than that expected from the differential rate of synthesis of the protein. We have detected no such activity in extracts of bacteria synthesizing pre-F-IF.

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