Open AccessHLA antigen-related restriction of T lymphocyte cytotoxicity to Epstein-Barr virus.
Author(s) -
Ihor S. Misko,
Denis J. Moss,
J. H. Pope
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.7.4247
Subject(s) - cytotoxic t cell , antigen , cytotoxicity , human leukocyte antigen , epstein–barr virus , biology , virology , pan t antigens , lymphoblast , virus , lymphocyte , immunology , t lymphocyte , microbiology and biotechnology , cell culture , in vitro , genetics
The specificity of cytotoxic T cells generated in Epstein-Barr virus (EBV)-infected lymphocyte cultures was investigated, using a 51Cr release assay. Potent cytotoxic T cells with preferred specificity directed to antigens expressed on autologous lymphoblastoid cell line (LCL) target cells were present in 14-day cultures of lymphocytes from EBV-seropositive donors and not from seronegative donors. Moreover, the cytotoxic patterns obtained with a panel of HLA antigen-related and unrelated LCL target cells, supported by unlabeled target inhibition tests, strongly indicate that T cell cytotoxicity to EBV is restricted to HLA antigens.