Open AccessEpstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping.
Author(s) -
Timothy R. Dambaugh,
Christopher E. Beisel,
Mary Hummel,
Walter E. King,
Susan M. Fennewald,
Andrew Cheung,
Mark Heller,
Nancy RaabTraub,
Elliott Kieff
Publication year - 1980
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.77.5.2999
Subject(s) - bamhi , ecori , hindiii , microbiology and biotechnology , pbr322 , dna , molecular cloning , biology , restriction map , genetics , chemistry , virology , restriction enzyme , plasmid , gene , peptide sequence
Two of the Sal I fragments and all of the internal BamHI fragments (with the exception of BamHI c, a 0.6 x 10(6) dalton fragment) of Epstein-Barr virus (EBV) DNA have been cloned in pBR322. The termini and other parts of the DNA (including the EcoRI fragment which contains BamHI c) have been cloned as EcoRI fragments in bacteriophage Charon 4A. The cloned DNAs have been used to derive a complete map of the BamHI fragments of EBV DNA and to align the BamHI, EcoRI, HindIII, and SalI cleavage sites in EBV DNA.