Open AccessDifferentiation of 3T3-L2 fibroblasts into adipose cells in bromodeoxyuridine-suppressed cultures.
Author(s) -
Thomas Russell
Publication year - 1979
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.76.9.4451
Subject(s) - bromodeoxyuridine , adipose tissue , deoxyuridine , 3t3 l1 , biology , dna synthesis , 3t3 cells , microbiology and biotechnology , cell culture , dna , chemistry , cell growth , endocrinology , adipocyte , biochemistry , transfection , genetics
Growth of 3T3-L2 fibroblasts in medium containing 5-bromo-2'-deoxyuridine prevents the spontaneous cytodifferentiation of these cells into adipose cells. Treatment of bromodeoxyuridine-suppressed cultures at confluence with 1-methyl-3-isobutylxanthine, a promoter of the adipose conversion, results in differentiation of the fibroblasts into adipose cells but the extent of the conversion is less than that seen in cultures not treated with bromodeoxyuridine. 1-Methyl-3-isobutylxanthine appears to function without altering the amount of bromodeoxyuridine present in DNA.