Open AccessPartial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.
Author(s) -
R. P. Ambler,
G. K. Scott
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.8.3732
Subject(s) - escherichia coli , plasmid , peptide sequence , biology , amino acid , sequence (biology) , gene , dna , plasmid preparation , enterobacteriaceae , recombinant dna , biochemistry , chemistry , microbiology and biotechnology , pbr322
Direct studies of the amino acid sequence of an Escherichia coli plasmid-coded penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) are in complete agreement with results derived from the translation of the DNA sequence of a related plasmid, apart from a single amino acid substitution. This penicillinase from a Gram-negative bacterium shows 30-35% identity with functionally similar enzymes from Gram-positive bacteria. This paper should be read in conjunction with the report of the DNA sequence of the gene.