Studies on the murine Ss protein: demonstration that the Ss protein is functionally the fourth component of complement. | Zendy

Michael C. Carroll | Zendy; J D Capra | Zendy

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Studies on the murine Ss protein: demonstration that the Ss protein is functionally the fourth component of complement.

Author(s) -

Michael C. Carroll,

J D Capra

Publication year - 1978

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.75.5.2424

Subject(s) - titer , complement (music) , ascitic fluid , microbiology and biotechnology , chemistry , complement system , biology , antibody , biochemistry , immunology , medicine , gene , ascites , complementation , phenotype

This report describes a simple and reproducible assay for the fourth component of murine complement (C4). Utilizing this assay, experiments are described which conclusively demonstrate that the murine Ss protein (serum substance) is functional C4 and that ascitic fluid may be used as a source for isolating Ss. The evidence that Ss is functional C4 is: (i) pretreatment of mouse plasma with F(ab')2 anti-Ss completely abrogated the C4 hemolytic titer; and (ii) in the isolation of the Ss protein from ascitic fluid, Ss immunochemical activity and C4 functional activity coeluted in each step of a four-step purification procedure.

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