Open AccessTen-nanometer filaments of hamster BHK-21 cells and epidermal keratin filaments have similar structures.
Author(s) -
Peter M. Steinert,
Steven B. Zimmerman,
Judith M. Starger,
Robert D. Goldman
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.12.6098
Subject(s) - keratin , protein filament , intermediate filament , baby hamster kidney cell , hamster , biophysics , protein subunit , ultrastructure , biology , crystallography , chemistry , cytoskeleton , microbiology and biotechnology , biochemistry , anatomy , cell , paleontology , gene
The 10-nm filaments of baby hamster kidney (BHK-21) cells, when examined either in the form of native filament caps or polymerized in vitro, are long tubes of protein 8--10 nm in diameter. They contain about 42% alpha-helix, which, on the basis of x-ray diffraction data, is arranged in a coiled-coil conformation characteristic of proteins of the alpha type. The known structural properties such as morphology, dimensions, subunit composition, and ultrastructure of this fibrous protein are very similar to those of the mammalian epidermal keratin filament, to which it may therefore be related.