Open AccessDemonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster.
Author(s) -
P Maróy,
Roger D. Dennis,
C. Beckers,
Becky A. Sage,
John D. O’Connor
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.12.6035
Subject(s) - drosophila melanogaster , dissociation constant , cytosol , ligand (biochemistry) , incubation , cytoplasm , in vivo , receptor , cell culture , nuclear receptor , biochemistry , biology , biophysics , chemistry , transcription factor , enzyme , genetics , gene
Incubation of the high-speed supernatant from the Kc cell line of Drosophila melanogaster with [3H]ponasterone A results in significant binding of the ligand as determined by gel filtration and dextran-coated charcoal binding assays. In vivo exposure of the Kc line to [3H]ponasterone A for 30 min results in a marked binding of the ligand by a KCl-soluble nuclear extract. With both the cytosol and nuclear preparations the binding has specificity and a low dissociation constant (3 X 10(-9) M). In addition the labeling of the nuclear preparation exhibits a saturation at approximately 7 X 10(-10) M which probably reflects the molar concentration of cytoplasmic receptors.