Distribution of yeast fatty acid synthetase subunits: three-dimensional model of the enzyme. | Zendy

Felix Wieland | Zendy; E.A. Siess | Zendy; Lauren Renner | Zendy; Cornelia VERFÜRTH | Zendy; Fréderic Lynen | Zendy

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Distribution of yeast fatty acid synthetase subunits: three-dimensional model of the enzyme.

Author(s) -

Felix Wieland,

E.A. Siess,

Lauren Renner,

Cornelia VERFÜRTH,

Fréderic Lynen

Publication year - 1978

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.75.12.5792

Subject(s) - biochemistry , enzyme , yeast , immunodiffusion , fatty acid , antibody , protein subunit , centrifugation , biology , differential centrifugation , chemistry , microbiology and biotechnology , immunology , gene

Rabbit and goat antibodies against the isolated alpha and beta subunits of yeast fatty acid aynthetase were raised and characterized. The purified IgG fractions were studied as to their capability to precipitate their antigens and the holoenzyme and to inhibit the partial reactions involved in overall fatty acyl-CoA synthesis. The specificity of the antibodies was investigated by immunodiffusion and by immunotitration. Native enzyme was crosslinked with each of the antibodies, and dimeric and oligomeric groups of IgG-crosslinked fatty acid synthetase molecules were isolated by sucrose density gradient centrifugation. Electron microscopic investigation of the crosslinked material as well as other data led us to suggest a three-dimensional model of yeast fatty acid synthetase.

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