A photo-CIDNP study of the interaction of oligonucleotides with gene-5 protein of bacteriophage M13. | Zendy

G.J. Garssen | Zendy; Robert Kaptein | Zendy; J.G.G. Schoenmakers | Zendy; C. W. Hilbers | Zendy

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A photo-CIDNP study of the interaction of oligonucleotides with gene-5 protein of bacteriophage M13.

Author(s) -

G.J. Garssen,

Robert Kaptein,

J.G.G. Schoenmakers,

C. W. Hilbers

Publication year - 1978

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.75.11.5281

Subject(s) - cidnp , oligonucleotide , bacteriophage , dna , quenching (fluorescence) , biophysics , chemistry , polarization (electrochemistry) , gene , biology , biochemistry , fluorescence , physics , escherichia coli , quantum mechanics

It is shown that photo-CIDNP effects (CIDNP, chemically induced dynamic nuclear polarization) can be generated in the 360-MHz proton NMR spectrum of gene-5 protein from bacteriophage M13. This technique is used to determine the number of tyrosyl residues at the surface of the protein and to assign the resonances from the 3,5-ring protons of these residues. The DNA-binding site of the protein is investigated by formation of complexes with oligonucleotides. Complex formation leads to shifting and/or quenching of the photo-CIDNP emission signals of the surface tyrosines, implying that they are involved in DNA-protein interaction. These experiments are complemented by studying the complex formation of Lys-Tyr-Lys to poly(A).

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