Open AccessDNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.
Author(s) -
M.I. Baldi,
Emilio Mattoccia,
Glauco P. TocchiniValentini
Publication year - 1978
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.75.10.4873
Subject(s) - xenopus , dna supercoil , dna , biology , oocyte , simian , histone , virus , microbiology and biotechnology , virology , embryo , genetics , dna replication , gene
A purified system is described for the introduction of negative supercoils into simian virus 40 DNA. The system consists of histones H2A, H2B, H3, and H4, DNA-relaxing enzyme, and a purified factor from Xenopus laevis stage 6 oocyte nuclei. The nuclei are prepared en masse by the technique of F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa [(1978) Chromosoma 60, 299-308]. The supercoiled simian virus 40 DNA prepared by this method is indistinguishable from simian virus 40 supercoiled DNA prepared from infected monkey cells.