Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells. | Zendy

Daniel G. Tenen | Zendy; H S Garewal | Zendy; Lora L. Haines | Zendy; J. Hudson | Zendy; V Woodard | Zendy; Susan Light | Zendy; David M. Livingston | Zendy

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Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells.

Author(s) -

Daniel G. Tenen,

H S Garewal,

Lora L. Haines,

J. Hudson,

V Woodard,

Susan Light,

David M. Livingston

Publication year - 1977

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.74.9.3745

Subject(s) - simian , virus , biology , antigen , virology , polyacrylamide gel electrophoresis , sodium dodecyl sulfate , microbiology and biotechnology , cell culture , chemistry , biochemistry , enzyme , genetics

Simian virus 40 tumor antigen can be isolated in a highly purified state from the nuclei ofSV80 cells, a continuous line of simian virus 40-transformed human fibroblasts. A five-step purification method was used. Its apparent molecular weight (in sodium dodecyl sulfate/polyacrylamide gels) is approximately 90,000-94,000. It contains a detectable amino-terminal residue.

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