Open AccessShape of the 50S subunit of Escherichia coli ribosomes.
Author(s) -
H. B. Stuhrmann,
Michel H. J. Koch,
R. Parfait,
J. Haas,
K. Ibel,
Robert R. Crichton
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.6.2316
Subject(s) - ribosome , protein subunit , 50s , extrapolation , escherichia coli , chemistry , neutron scattering , electron microscope , scattering , crystallography , physics , biochemistry , optics , mathematics , rna , mathematical analysis , gene
Extrapolation of a series of low-angle neutron scattering curves to infinitely high contrast gives a scattering function IC(kappa) which is dependent on the shape of the solute molecule. For the 50S subunit of E. coli ribosomes, the first part of the structure determination by neutron scattering, namely the determination of the molecular shape from IC(kappa), is reported. The result is in good agreement with models of the 50S subunit determined by electron microscopy.