Open AccessAdhesive specificity of juvenile rat and chicken liver cells and membranes.
Author(s) -
Björn Öbrink,
Mark S. Kuhlenschmidt,
Saul Roseman
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.3.1077
Subject(s) - collagenase , membrane , heterologous , cell , homologous chromosome , cell membrane , biology , adhesion , cell adhesion , liver cell , cell type , microbiology and biotechnology , biochemistry , chemistry , medicine , enzyme , organic chemistry , gene
Liver cells, isolated from either juvenile rats or chickens by a collagenase perfusion technique, reaggregated when maintained in suspension. The cells exhibited marked adhesive specificity; when suspensions contained both cell types, the aggregates consisted primarily of either rat or chicken cells. Adhesive specificity was also observed with plasma membrane fractions isolated from rat liver homogenates, and with comparable fractions from chicken liver. These membranes stimulated aggregation of the homologous but not the heterologous cell type. Other membrane fractions had little or no effect on the aggregation of the homologous cell type. These and other properties of the liver cell and membrane preparations suggest that biochemical studies on cell-cell recognition and adhesion can most effectively be conducted with cells from juvenile and adult animals.