Open AccessComparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.
Author(s) -
D. Lohr,
Jeffry L. Corden,
Kelly Tatchell,
R T Kovacic,
Kensal E. van Holde
Publication year - 1977
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.74.1.79
Subject(s) - micrococcal nuclease , chromatin , nuclease , hela , dna , biology , yeast , protein subunit , microbiology and biotechnology , ovalbumin , biochemistry , genetics , nucleosome , cell , gene , immune system
We have compared the chromatin subunit structure of yeast, HeLa, and chicken erythrocyte by analyzing the DNA fragments produced by in situ digestion with staphylococcal nuclease (EC 3.1.4.7) and DNase I(EC 3.1.4.5). The repeat size of the chromatin varies among (and within two of) the three organisms but the size and the structure of the most nuclease-resistant "core" of the repeat is the same. Thus, the interspecies differences in repeat size are due to different lengths of nuclease-sensitive "spacer" DNA between the cores. There also seems to be a difference in the manner of spacing of cores; the transcriptionally active (yeast and HeLa) chromatins have spacings of variable length while the transcriptionally inactive (chicken erythrocyte) has a more regular spacing of cores.