Decreased fidelity of DNA polymerase activity isolated from aging human fibroblasts. | Zendy

Stuart Linn | Zendy; M. Kairis | Zendy; R. Holliday | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Decreased fidelity of DNA polymerase activity isolated from aging human fibroblasts.

Author(s) -

Stuart Linn,

M. Kairis,

R. Holliday

Publication year - 1976

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.73.8.2818

Subject(s) - polymerase , dna polymerase , nucleotidyltransferase , dna , microbiology and biotechnology , biology , biochemistry , chemistry , gene , rna

DNA polymerase (deoxynucleosidetriphosphate: DNA nucleotidyltransferase, EC 2.7.7.7 or DNA nucleotidyltransferase) activity, isolated from late and early passage cells of the diploid human fibroblast line, MRC-5, was compared. The level of activity dropped with increasing passage. In addition, when the fidelity of polymerization was monitored with four synthetic templates under a variety of conditions, it was observed that the enzyme from late passage cells was more error-prone. The possible relation of these observations to "senescence" of the fibroblasts is discussed.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research