Open AccessHuman papilloma virus DNA: physical mapping and genetic heterogeneity.
Author(s) -
Lutz Gissmann,
Harald zur Hausen
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.4.1310
Subject(s) - ecori , restriction enzyme , cleavage (geology) , dna , microbiology and biotechnology , agarose gel electrophoresis , biology , agarose , restriction digest , restriction map , genetics , base sequence , paleontology , fracture (geology)
The molecular weight of three preparations of human papilloma virus DNA derived from different plantar warts was determined by agarose gel electrophoresis or electron microscopic contour length measurement. It was found to amount to approximately 4.9 X 10(6). Analysis of this DNA after sequential digestion by four different restriction endonucleases (EcoRI, Bam, Hind II, and Hind III) permitted physical mapping of the cleavage sites. Two of the three DNA preparations revealed an identical cleavage pattern, whereas the third one contained two additional cleavage sites.