Open AccessStucture of the amino-acid accepting 3'-end of high-molecular-weight eggplant mosaic virus RNA.
Author(s) -
JeanPaul Briand,
K. Richards,
J.P. Bouley,
JeanFrançois Witz,
L. Hirth
Publication year - 1976
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.73.3.737
Subject(s) - rna , rnase p , oligonucleotide , nucleotide , valine , amino acid , transfer rna , biology , biochemistry , turnip yellow mosaic virus , rnase h , nucleic acid , nucleic acid sequence , gene
The sequence of the first 59 nucleotides from the 3'-OH terminus of high-molecular-weight eggplant mosaic virus RNA has been determined by standard radio-chemical techniques. The fragment was identified among the products of partial T1 RNase digestion by making use of the reverse migration, at pH 2.5, of the 3'-OH terminal oligonucleotide. No abnormal bases were found. A model of secondary structure may be constructed for this fragment, which is known to fix valine in the presence of valyl-tRNA synthetase. Its relation to the structures of genuine tRNAs and to the 3'-OH termini of other viral RNAs that also accept amino acids is discussed.