Open AccessNucleotide sequence of the rightward operator of phage lambda.
Author(s) -
Tom Maniatis,
Andrea Jeffrey,
Dennis G. Kleid
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.3.1184
Subject(s) - lambda phage , operator (biology) , bacteriophage , base pair , sequence (biology) , dna , lambda , genetics , nucleic acid sequence , biology , nucleotide , repressor , physics , gene , escherichia coli , transcription factor , optics
The sequence of 72 base pairs of the rightward operator (O-R) of bacteriophage lambda is presented as determined with simple and rapid methods for direct DNA sequencing. The sequence of an operator mutant is also described. The methods are of general use in sequencing DNA fragments with unique 5' ends up to 50 base pairs in length. Previous experiments have shown that this operator contains multiple sites recognized by the lambda phage repressor. We believe we have identified three of these sites.