Open AccessIsolation of the bacteriophage lambda A-gene protein.
Author(s) -
Andrew Becker,
Marvin Gold
Publication year - 1975
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.72.2.581
Subject(s) - bacteriophage , lambda phage , lambda , gene , gene product , dna , microbiology and biotechnology , biology , phagemid , in vitro , chemistry , genetics , escherichia coli , gene expression , physics , optics
An in vitro assay for measuring the activity of the phage lambda A-gene product has been developed. The assay is based on the observation that A-donor extracts complement A minus extracts for packaging of exogenous immature lambda DNA into phage particles. A partial purification of the A-gene product activity using this assay is presented. A method is suggested by which this A protein-dependent in vitro system might be manipulated to analyze the mechanism of reforming the lambda cohesive termini during chromosome assimilation into phage precursors.