Isolation of the bacteriophage lambda A-gene protein. | Zendy

A Becker | Zendy; Marvin Gold | Zendy

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Isolation of the bacteriophage lambda A-gene protein.

Author(s) -

A Becker,

Marvin Gold

Publication year - 1975

Publication title -

proceedings of the national academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.011

H-Index - 771

eISSN - 1091-6490

pISSN - 0027-8424

DOI - 10.1073/pnas.72.2.581

Subject(s) - bacteriophage , lambda phage , lambda , gene , gene product , dna , microbiology and biotechnology , biology , phagemid , in vitro , chemistry , genetics , escherichia coli , gene expression , physics , optics

An in vitro assay for measuring the activity of the phage lambda A-gene product has been developed. The assay is based on the observation that A-donor extracts complement A minus extracts for packaging of exogenous immature lambda DNA into phage particles. A partial purification of the A-gene product activity using this assay is presented. A method is suggested by which this A protein-dependent in vitro system might be manipulated to analyze the mechanism of reforming the lambda cohesive termini during chromosome assimilation into phage precursors.

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