Open AccessSpecificity of mRNA Binding Factor in Eukaryotes
Author(s) -
Stuart M. Heywood
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.4.1782
Subject(s) - ribosome , messenger rna , reticulocyte , biology , ribosomal binding site , translation (biology) , globin , initiation factor , microbiology and biotechnology , biochemistry , chemistry , rna , hemoglobin , gene
It has been shown previously that ribosomal factors removed from chick muscle ribosomes by a high salt wash are required for both the binding of muscle mRNA to ribosomes and mRNA-directed synthesis of myosin on reticulocyte ribosomes. These factors can be separated into several components on DEAE-cellulose. A factor eluting between 0.18 and 0.25 M KCl is responsible for binding mRNA to the ribosome. This binding factor shows specificity in the recognition of mRNA, as has been demonstrated by the preferential binding of muscle and globin mRNA to ribosomes which contain their respective binding factors. Similarly, globin mRNA is preferentially translated when reticulocyte factors are present on the ribosome.