Open AccessIdentification of the Cytokinin-Active Ribonucleosides in Pure Escherichia coli tRNA Species
Author(s) -
John K. Bartz,
Dieter Söll,
William Burrows,
Folke Skoog
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.3.1448
Subject(s) - escherichia coli , transfer rna , identification (biology) , biology , biochemistry , botany , rna , gene
The distribution of the two cytokinin-active ribonucleosides 6-(3-methyl-2-butenylamino)-9-β-D-ribofuranosylpurine (1) and 6-(3-methyl-2-butenylamino)-2-methylthio-9-β-D-ribofuranosylpurine (2) in tRNAPhe , tRNAUUG Leu , tRNAUCR Ser , tRNATyr , tRNACys , and tRNA2 Trp from stationary phaseEscherichia coli has been studied. Compound 2 predominated in all these species except tRNAPhe , in which compound 1 was greatly in excess. Compound 1 was present in relatively small amounts in tRNAUUG Leu , tRNACys , and tRNA2 Trp , and was not detected in tRNAUCR Ser and tRNATyr . Cells from the early logarithmic phase had the highest cytokinin content in their tRNA. Stationary phase tRNA had a slight increase in the amount of 1, which may be explained by the appearance of the new tRNAPhe . No differences were observed between the chromatographic patterns of tRNAs from early-, mid-, and latelogarithmic phases.