Open AccessLow Temperature and Chloramphenicol Induction of Respiratory Deficiency in a Cold-Sensitive Mutant of Saccharomyces cerevisiae
Author(s) -
Paul O. Weislogel,
Ronald A. Butow
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.67.1.52
Subject(s) - saccharomyces cerevisiae , mutant , chloramphenicol , strain (injury) , biology , yeast , cold sensitivity , biochemistry , mitochondrial dna , cytoplasm , respiration , chemistry , microbiology and biotechnology , gene , antibiotics , anatomy , botany
A cold-sensitive mutant of a haploid strain ofSaccharomyces cerevisiae has been isolated by selection for impaired growth at 18° on nonfermentable carbon sources. Growth of the mutant on glucose or galactose at either 28 or 18° is similar to that of the parental strain. The cold-sensitive strain is highly mutable to a cytoplasmic petite when grown at 18°, or when grown at 28° in the presence of 4 mg/ml of chloramphenicol. Cold sensitivity is not observed in the conversion of promitochondria to mitochondria. We conclude that mitochondrial protein synthesis is required for maintaining the stability of the mitochondrial genome.