Open AccessNeutralization of Sensitized Virus by Purified Components of Complement
Author(s) -
Charles A. Daniels,
Tibor Borsos,
Herbert J. Rapp,
Ralph Snyderman,
Abner Louis Notkins
Publication year - 1970
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.65.3.528
Subject(s) - neutralization , virus , herpes simplex virus , limiting , antibody , chemistry , immunoglobulin g , virology , biology , immunology , mechanical engineering , engineering
Herpes simplex virus which had been sensitized with immunoglobulin M antibody was neutralized by serum deficient in the fifth and sixth components of complement (C) but not by serum deficient in the fourth component C (C4). The sequential addition of the functionally purified components of C showed that the activated first component of C (C1[unk]) failed to neutralize sensitized virus. However, in the presence of an optimal concentration of C1[unk], the addition of C4 resulted in neutralization. The amount of virus neutralized was dependent upon the concentration of immunoglobulin M used to sensitize the virus and the concentration of C1[unk] and C4. The addition of the second component of C (C2) to reaction mixtures containing an optimal concentration of C1[unk] and a limiting concentration of C4 resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C2. The addition of the third component of C (C3) to reaction mixtures containing an optimal concentration of C1[unk] and limiting concentrations of C4 and C2 also resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C3.