Open AccessDEMONSTRATION OF DELAYED HYPERSENSITIVITY TO SOLUBLE ANTIGENS OF CHEMICALLY INDUCED TUMORS BY INHIBITION OF MACROPHAGE MIGRATION
Author(s) -
Barry R. Bloom,
Boyce Bennett,
Herbert F. Oettgen,
Elisabeth P. McLean,
Lloyd J. Old
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.64.4.1176
Subject(s) - macrophage migration inhibitory factor , antigen , macrophage , in vitro , in vivo , sensitization , delayed hypersensitivity , lymph node , chemistry , immunology , transplantation , microbiology and biotechnology , biology , medicine , biochemistry , cytokine
Sensitization to soluble antigens of chemically induced tumors is demonstrable in syngeneic strain 13 (inbred) guinea pigs by specific inhibition of macrophage migration. According to their capacity to (a ) inhibit the migration of peritoneal cells from sensitized strain 13 donors or (b ) release migration-inhibitory factor from their lymph node cells, the soluble antigens of three different chemically induced tumors did not cross react. This is in keeping with the absence of cross-reactivity among the same three tumors in previous tests of transplantation resistance or delayed hypersensitivityin vivo . Thus, inhibition of macrophage migration may provide a feasible assayin vitro for use in defining these soluble antigens further by physicochemical methods.