Open AccessMICROELECTRODE STUDIES ON THE MEMBRANE PROPERTIES OF ISOLATED MITOCHONDRIA, II. ABSENCE OF A METABOLIC DEPENDENCE
Author(s) -
Joseph T. Tupper,
Henry Tedeschi
Publication year - 1969
Publication title -
proceedings of the national academy of sciences of the united states of america
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.011
H-Index - 771
eISSN - 1091-6490
pISSN - 0027-8424
DOI - 10.1073/pnas.63.3.713
Subject(s) - microelectrode , membrane potential , mitochondrion , biophysics , membrane , inner mitochondrial membrane , chemistry , swelling , biology , biochemistry , materials science , electrode , composite material
Mitochondrial membrane potentials and resistances have been determined under various metabolic conditions by the use of piezoelectric-driven microelectrodes. The mean state 3 potentials (about +19 mv) are higher than the potentials in state 4 (about +9 mv). DNP in uncoupling concentrations or KCN have no significant effect on membrane resistance or potential. Previous experiments have shown the potential to be sensitive to mitochondrial swelling and have suggested that it depends predominantly on anionic distribution.